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. 2004 Apr 6;101(16):6237–6242. doi: 10.1073/pnas.0401413101

Fig. 1.

Fig. 1.

ND2 is a Src unique domain-interacting protein. (A) Schematic diagram illustrating the domain structure of ND2, clones isolated from the yeast two-hybrid screen, and recombinant GST-tagged fusion proteins. (B) Blot of ND2–GST fusion proteins probed with biotinylated Src unique domain followed by SA-HRP. (C) Blot of ND2.1–GST probed with biotinylated domains of Src and Fyn followed by SA-HRP. (D) Immunoblots of coimmunoprecipitates from brain homogenate probed with anti-ND2, anti-Src, or anti-Fyn. Nonspecific IgG was used as a negative control for immunoprecipitation. In these experiments, the cell lysates were prepared by using nondenaturing conditions; under denaturing conditions, we found no coimmunoprecipitation of Src by anti-ND2 or of ND2 by anti-Src (not shown). (E) Immunoblot of coimmunoprecipitates from cultured Src+/+ and Src-/- fibroblasts probed with anti-ND2. Nonspecific IgG was used as a negative control for immunoprecipitation, and immunoblotting of ND2 protein from both cell lines was used as a positive control.