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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Eur J Immunol. 2014 Jan 13;44(3):842–855. doi: 10.1002/eji.201343755

Figure 1.

Figure 1

IL-13Rα1 is expressed on various cell types and forms a heteroreceptor with the IL-4Rα chain. Splenic cells from IL-13Rα1+/+, IL-13Rα1−/− and IL-13Rα1-GFP reporter neonatal and adult C57BL/6 mice were stained for basophil, mast cell, dendritic cell, and macrophage markers. (A) Cells from IL-13Rα1+/+ and IL-13Rα1−/− mice were also stained with an anti-IL-13Rα1 monoclonal antibody and 7AAD, and IL-13Rα1 expression was analyzed on live cells (7AAD−), gated on the indicated cell type. (B) Splenic cells from IL-13Rα1-GFP reporter neonatal and adult C57BL/6 mice were stained with 7AAD but not with anti-IL-13Rα1 monoclonal antibody and IL-13Rα1 expression was determined by analysis of GFP expression on live cells (7AAD−) gated on the indicated cell type. Numbers represent the percentage of cells expressing IL-13Rα1. Results obtained with cells pooled from 2 mice are shown and are representative of 4 independent experiments. (C) Expression of IL-13Rα1 in intestinal, lung and liver cells of neonatal and adult IL-13Rα1+/+ BALB/c mice as determined by western blot using an anti-IL-13Rα1 mAb. Intestinal epithelial cells from adult and neonatal IL-13Rα1−/− mice were included as a negative control. Results show samples from 2 mice and the data are representative of 3 independent experiments. (D) Immunoprecipitation (IP) of the heteroreceptor by anti-IL-4Rα and western blotting (WB) of IL-13Rα1 with anti-IL-13Rα1 Ab (top). For control purposes, equivalent amounts of lysate were subjected to IP with anti-β-actin antibody and blotted with the same antibody. In the bottom panel, the heteroreceptor immunoprecipitated with anti-IL-13Rα1 and IL-4Rα was blotted with anti-IL-4Rα Ab. (B, C) β-actin was used as a loading control. Intestinal cells from IL-13Rα1−/− BALB/c mice were included as negative control. The data are representative of three independent experiments obtained with cells pooled from 2 mice per experiment.