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HSP60 knockdown causes ROS accumulation in HEK293 cells. (A) HSP60 abundance was assessed two days after knockdown with HSP60 Trilencer-27 Human siRNA; control cells were transfected with a siRNA not targeting any known gene in the human genome. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as loading control. (B) Cellular ROS levels in HSP60 knockdown cells (left), knockdown controls (middle), and knockdown controls in the absence of fluorophore (right).
