Figure 5.

AKT inhibition or ROS-induced senescence is mediated by HMGA2 up-regulation and colocalization to SAHF. Leiomyoma cells were transiently transfected with siRNA to HMGA2 or a scrambled control siRNA. Cells were then treated with MK-2206 (2 μM), H₂O₂ (100 μM), NAC, and DOX (0.2 μg/mL). A, Immunofluorescent staining of HMGA2 was done and visualized. Cells were costained with DAPI. B, Percentage of cells with heterochromatin foci was calculated. C, Levels of HMGA2 at the protein level were measured by Western blot analysis to verify silencing efficacy (n = 3). *, P < .05; **, P < .01; ***, P < .001.