Table 1. Induction of mycorrhiza-specific phosphate transporters in plant species of different taxonomic positions.
| Promoter region fused to GUS
|
|||
|---|---|---|---|
| Plants studied | StPT3 | MtPT4 | OsPT11 |
| Eudicots | |||
| Asteridae | |||
| Solanales | |||
| Solanum tuberosum | +, G.m., P | +, G.m., P | —, G.m., P |
| Petunia hybrida | +, G.m.,I | ||
| Apiales | |||
| Daucus carota | +, G.L., P | ||
| Rosidae | |||
| Fabales | |||
| Lotus japonicus | +, G. mos., I | ||
| Medicago truncatula | +, G.mos., I | +, G.mos., I | —, G.mos., I |
Potato (S. tuberosum) and carrot (D. carota) transgenic hairy roots. L. japonicus, and M. truncatula composite plants with transgenic hairy roots and transgenic petunia (Petunia × hybrida) were generated by means of agrobacterial transformation with constructs carrying promoter regions of mycorrhiza-specific phosphate transporters from potato (StPTs; 1.72 Kb), M. truncatula (MtPT4; 0.87 Kb), and rice (O. sativa) (OsPT11; 0.8, 1.54, and 3.16 Kb) fused to the GUS reporter gene. Roots were inoculated with one of three AMF species: G.m., Gigaspora margarita; G.i., Glomus intraradices; and G. mos., Glomus mosseae. Mycorrhizal roots were stained for GUS activity. +, Positive GUS staining in root zones colonized by AMF; —, absence of GUS staining. P and I indicate formation of either Paris- or intermediate-type AM, respectively.