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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Methods Cell Biol. 2012;107:409–436. doi: 10.1016/B978-0-12-394620-1.00014-X

Figure 11. Pressure-polishing microforge.

Figure 11

This inverted microscope is equipped with a long-working distance 100× objective (Leica), a CPM-2 microforge kit (ALA Science), and a custom-designed pressure polishing accessory. Compressed air is passed through a regulator/filter (not shown) to provide a high-pressure (40 PSI) air stream. This stream is directed to the pipette being shaped by means of a four-way valve and a pipette holder equipped with a luer-lock connector (WPI Instruments). For a visual demonstration of the process, see (Johnson et al., 2008).