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. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: Rheumatology (Sunnyvale). 2012 Dec 26;2:113. doi: 10.4172/2161-1149.1000113

Figure 2.

Figure 2

rhTNF-α, AG-490 and rhTNF-α/febuxostat Increase TUNEL-Positive Hu-C. Normal human chondrocytes were grown in 3.8 cm2 microwell culture dishes and incubated either with rhTNF-α (1.5 ng/ml), AG-490 (50 μM) or rhTNF-α (1.5 ng/ml) plus febuxostat (fbx) (16μM) (n=3) for 24 hours. The total number of TUNEL-positive cells in at least 60 individual fields was enumerated. Panel A: TUNEL-positive chondrocytes with matching phase-contrast images. Panel B: The total number of TUNEL-positive chondrocytes in a representative field from the control groups and from each of the experimental groups is shown. There were 2 control groups: C-1 contained medium alone; C-2 contained medium plus DMSO at the concentration used to dissolve AG-490.