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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 30;0:135–142. doi: 10.1016/j.jchromb.2014.01.037

Figure 1.

Figure 1

Schematic representation of the platform for the simultaneous enrichment of fucosylated glycoproteins and the subsequent RPC fractionation of the captured proteins. When the switching valve (SV) A, B, and C were in 1, 3 and 6 positions, respectively, the equalized serum was injected onto the lectin columns by-passing the RPC column, followed by washing with the binding mobile phase (BMP) using pump A. Then, the eluting mobile phase for the LTA column was passed by keeping the SV-A position in 1 and changing the SV-B position to 4 and that of SV-C to 5, thus by-passing the AAL column and passing through the RPC column. This was followed by washing, eluting and re-equilibrating the RPC column using the mobile phase from pump B. Then, the AAL column was eluted by changing the SV-A position to 2, keeping the SV-B position in 3 and SV-C position in 5. This was again followed by washing, eluting and re-equilibrating the RPC column and re-equilibrating the lectin columns with the BMP.