Fig. 2.

Condition optimisation for NMR studies of T-STAR constructs. ¹⁵N–¹H HSQC spectra of T-STAR constructs. (A) STAR domain in 20 mM sodium phosphate pH 6.5, 50 mM NaCl, 30 °C. (B) KH-QUA2 in 20 mM sodium phosphate pH 6.5, 50 mM NaCl, 30 °C. (C) KH in 10 mM Tris–HCl pH 6.5, 50 mM NaCl, 30 °C. (D) Overlay of the ¹⁵N–¹H HSQC spectra of T-STAR KH-QUA2 (black) and KH (red).