Skip to main content
. Author manuscript; available in PMC: 2015 Mar 13.
Published in final edited form as: Cell. 2014 Mar 13;156(6):1179–1192. doi: 10.1016/j.cell.2014.01.014

Figure 2. GFAT1 is a direct target of Xbp1s.

Figure 2

(A) A conserved DNA motif, similar to the UPRE, was identified in the GFAT1 promoter from different species.

(B) GFAT1 promoter was stimulated by Xbp1s overexpression. The GFAT1 promoter activity was measured by a luciferase assay upon Xbp1s co-transfection in HEK293T cells. N = 3 for each group.

(C) Xbp1s was associated with A ChIP assay was conducted in C2C12 cells after Xbp1s overexpression. PCR amplification was performed using primers spanning the UPRE or from a distal region in the GFAT1 promoter. The triangles indicate increasing amounts of immunoprecipitated DNA for PCR reaction.

(D) GFAT1 transcription was stimulated by Xbp1s in vitro. qPCR was conducted to quantify relative mRNA levels of GFAT1 after Xbp1s overexpression in NRVM. N = 6.

(E) GFAT1 protein levels were elevated by Xbp1s induction. N = 3–4. Data are represented as mean ± SEM. *, p < 0.05.

See also Figure S2.