Table 1.
Oligonucleotide Primers for Amplification of 22 Human Mitochondrial tRNAs
| Locus | Starting | Ending | Length (bp) | Number of Variants | Primer22 | Primer Sequence 5′‐3′ |
|---|---|---|---|---|---|---|
| tRNAPhe | 577 | 647 | 71 | 5 | 1F/1R | CTCCTCAAAGCAATACACTG/TGCTAAATCCACCTTCGACC |
| tRNAVal | 1602 | 1670 | 69 | 5 | 2F/2R | CGATCAACCTCACCACCTCT/TGGACAACCAGCTATCACCA |
| tRNALeu(UUR) | 3230 | 3304 | 75 | 6 | 4F/4R | AAATCTTACCCCGCCTGTTT/AGGAATGCCATTGCGATTAG |
| tRNAIle | 4263 | 4331 | 69 | 10 | 6F/6R | TGGCTCCTTTAACCTCTCCA/AAGGATTATGGATGCGGTTG |
| tRNAGln | 4329 | 4400 | 72 | 12 | 6F/6R | As above |
| tRNAMet | 4402 | 4469 | 68 | 6 | 6F/6R | As above |
| tRNATrp | 5512 | 5579 | 68 | 8 | 7F/8R | ACTAATTAATCCCCTGGCCC/ACCTAGAAGGTTGCCTGGCT |
| tRNAAla | 5587 | 5655 | 69 | 6 | 8F/8R | CTAACCGGCTTTTTGCCC/ACCTAGAAGGTTGCCTGGCT |
| tRNAAsn | 5657 | 5729 | 73 | 5 | 8F/8R | As above |
| tRNACys | 5761 | 5826 | 66 | 15 | 8F/8R | As above |
| tRNATyr | 5826 | 5891 | 66 | 4 | 8F/8R | As above |
| tRNASer(UCN) | 7446 | 7514 | 69 | 3 | 11F/11R | ACGCCAAAATCCATTTCACT/CGGGAATTGCATCTGTTTTT |
| tRNAAsp | 7518 | 7585 | 68 | 6 | 11F/11R | As above |
| tRNALys | 8295 | 8364 | 70 | 7 | 12F/12R | ACGAGTACACCGACTACGGC/TGGGTGGTTGGTGTAAATGA |
| tRNAGly | 9991 | 10058 | 68 | 10 | 14F/15R | CCCACCAATCACATGCCTAT/AATTAGGCTGTGGGTGGTTG |
| tRNAArg | 10405 | 10469 | 65 | 5 | 15F/15R | TCTCCATCTATTGATGAGGGTCT/AATTAGGCTGTGGGTGGTTG |
| tRNAHis | 12138 | 12206 | 69 | 6 | 18F/18R | TATCACTCTCCTACTTACAG/AGAAGGTTATAATTCCTACG |
| tRNASer(AGY) | 12207 | 12265 | 59 | 11 | 18F/18R | As above |
| tRNALeu(CUN) | 12266 | 12336 | 71 | 5 | 18F/18R | As above |
| tRNAGlu | 14674 | 14742 | 69 | 5 | 21F/21R | GCATAATTAAACTTTACTTC/AGAATATTGAGGCGCCATTG |
| tRNAThr | 15888 | 15953 | 66 | 27 | 22F/23R | TGAAACTTCGGCTCACTCCT/GAGTGGTTAATAGGGTGATAG |
| tRNAPro | 15956 | 16023 | 68 | 6 | 22F/23R | TGAAACTTCGGCTCACTCCT/GAGTGGTTAATAGGGTGATAG |