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. 2014 Feb 28;3(1):e000622. doi: 10.1161/JAHA.113.000622

Figure 1.

Figure 1.

Klf4‐cKO mice grew up normally to the adulthood. A through C, Body weight (A), systolic and diastolic blood pressure (B), and heart rate (C) of Klf4‐cKO mice and control mice were measured at 11 weeks of age (n=12 for each genotype). B, The tops and bottoms of the squares indicate systolic and diastolic blood pressure, respectively. D through F, Expression of Klf4 in the aorta (D), colon (E), and blood (F) was determined by real‐time RT‐PCR in Klf4‐KO mice and control mice (n=4 for each genotype). *P<0.05 compared with control mice. G, Recombination of the Klf4loxP allele was examined by PCR in the blood of Klf4‐cKO mice (cKO) and control mice (Ct). H, Klf4 expression was examined by immunohistochemistry in the carotid arteries of Klf4‐cKO mice and control mice 3 days after injury. Klf4 expression was visualized by diaminobenzidine, and sections were counterstained with hematoxylin. Representative pictures are shown from 6 mice analyzed per genotype. Bar: 50 μm. Red arrowheads indicate the IEL. cKO indicates conditional knockout; IEL, internal elastic lamina; RT‐PCR, reverse‐transcription polymerase chain reaction.