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. 2014 Feb 28;3(1):e000622. doi: 10.1161/JAHA.113.000622

Figure 2.

Figure 2.

Expression of SMC differentiation markers was decreased in Klf4‐cKO mice after vascular injury. A, Expression of SM α‐actin and SM22α was examined by immunohistochemistry in the carotid arteries of Klf4‐cKO mice and control mice on day 3 and day 7 after ligation injury. Expression of SM α‐actin and SM22α was visualized by Vector Red alkaline phosphatase, and sections were counterstained with hematoxylin. Representative pictures are shown from 6 mice analyzed per genotype and from each time. Bar: 50 μm. B and C, Expression of SM α‐actin (B) and SM22α (C) was determined by real‐time RT‐PCR in the injured (Inj) and uninjured (Un) carotid arteries of Klf4‐cKO mice and control mice on day 7 after injury (n=5 per each genotype). *P<0.05 compared with uninjured arteries. cKO indicates conditional knockout; Ct, control; RT‐PCR, reverse‐transcription polymerase chain reaction; SMC, smooth muscle cell.