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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Science. 2013 Jul 19;341(6143):1238303. doi: 10.1126/science.1238303

Fig. 2. LINCS analysis reveals that targeting protein translation inactivates HSF1.

Fig. 2

A. Schematic representation of the LINCS analysis used to identify chemical and genetic modulators that are correlated with HSF1 inactivation. Pink represents genes whose levels increase and green represents genes whose levels decrease following shRNA mediated knockdown of HSF1. B. GSEA results of our HSF1 inactivation signature LINCS analysis. Perturbation signatures were rank-ordered by connectivity with the HSF1 inactivation signature and enrichment was determined for KEGG pathway gene sets and ATC chemical classes (see Materials and Methods for details). Normalized enrichment score (NES) of selected results are plotted (complete GSEA results are provided in table S4). C. Barcode plot of the connectivity score of all of the individual perturbations comprising the indicated enriched chemical or gene sets. The bagel plot in the center of the barcode plot summarizes the positive, negative and null (not connected) fractions for the indicated enriched class. All perturbations that are positively or negatively connected for the indicated enriched classes are shown. Total perturbations in each class are indicated on the right of the plot. Blue represents negatively connected and red represents positively connected classes of enriched perturbations.