Fig. 7.
Levels of 8-OHdG in BA21 ASD postmortem brain. (A) Representative images for immunohistochemistry of 8-OHdG in temporal lobe layer V pyramidal neurons. Pyramidal neurons were identified by a characteristic triangular morphology with a prominent apical dendrite pointing toward pial surface. Samples from 3 controls (C1, 2 yrs; C2, 4 yrs; C3, 5 yrs) and 3 ASD patients (A1, 3 yrs; A2: 3 yrs; A3, 7 yrs) were presented. Pyramidal neurons from boxed areas in C2 and A1 were shown in CP and AP. Scale bar, 20 μm. (B) Quantification of fluorescent intensity of cytoplasmic 8-OHdG per pyramidal neuron. Control, n=7; ASD, n=8. **, compared to controls, p<0.01. (C) Correlation analysis and non-linear regression of 8-OHdG fluorescent intensity with confounding factors (r, Spearman’s correlation coefficient; p, significance p value; R2, coefficient of determination).