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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Hippocampus. 2014 Feb;24(2):178–188. doi: 10.1002/hipo.22213

Figure 2.

Figure 2

Effect of acute administration of JWH-081 on ERK, CaMKIV and CREB phosphorylation levels in the hippocampus. (A) Western blot analyses were performed to determine the levels of pERK1/2 and ERK1/2 in hippocampal total extracts. β-actin was used as a loading control. Representative blots are shown for the hippocampal total extracts (n = 6 mice/group). (B) Hippocampal nuclear extracts from WT and KO mice treated with or without JWH-081 (1.25 mg/kg) for 30 or 60 min were processed for Western blot to analyze the levels of pCaMKIV, pCREB, CaMKIV and CREB. β-actin was used as a loading control. ***p < 0.001; **p < 0.01. (C) Hippocampal nuclear extracts from vehicle and SR pretreated C57BL/6J mice treated with or without JWH-081 (1.25 mg/kg) for 30 were processed for Western blot to analyze the levels of pCaMKIV (# and $ p< 0.05), pCREB (# and $ p< 0.01), CaMKIV and CREB. β-actin was used as a loading control. Representative blots are shown for the hippocampal nuclear extracts (n = 6 mice/group). One-way ANOVA with Bonferroni’s post hoc test was used for statistical analysis. The error bars represent SEM. ***p < 0.001 vs Vehicle (V); # vs Vehicle; $ vs JWH-081.