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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Exp Biol Med (Maywood). 2009 Jan 14;234(3):255–262. doi: 10.3181/0809-RM-275

Figure 1.

Figure 1

In vitro binding between p55 and merlin. (A) Schematic representation of NF2 protein (merlin) constructs used for the binding assays. (B) Coomassie blue stained SDS-PAGE showing purified recombinant proteins. MBP, lane 1; MBP-NF2-N, lane 2; and MBP-NF2-C, lane 3. (C) Western blot based detection of p55 recovered by the MBP-beads pull-down assay. Purified recombinant His-p55 expressed in bacteria was incubated with MBP-fusions of NF2 protein immobilized on the beads. Lane 4 represents the input His-p55 protein used as a positive control.