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. 2014 Feb 20;7:29–38. doi: 10.2147/JIR.S55672

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© 2014 Yoo et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Elevated tissue IL-4 expression and circulating OVA-specific IgE in OVA-challenged mice. (A and B) IL-4 and IFN-γ mRNA levels were measured by qRT-PCR. IL-4 increased over time, while IFN-γ decreased. (C) Serum OVA-specific IgE was measured by ELISA. Compared to nondetectable (ND) levels in sham-treated mice, there was a significant increase in IgE levels as early as day 1 in OVA-treated mice, with a continued increase over time. Data are presented as mean ± SEM of two (day 7) or four (days 1, 3) sham-treated mice, and five (days 1, 7) or nine (day 3) OVA-treated mice.

Abbreviations: DC, dendritic cell; ELISA, enzyme linked immunosorbent assay; OVA, ovalbumin; IFN, interferon; IL, interleukin; mRNA, messenger ribonucleic acid; ND, nondetectable; qRT-PCR, quantitative reverse transcription polymerase chain reaction; SEM, standard error of the mean.