Figure 7.

Systemic sensitization augments local sensitization alone in elicitation of an AD phenotype. (A) Diagrams comparing OVA sensitization and challenge protocols with (above; previously described) and without (below) IP sensitization. (B) Mice sensitized by EC route alone (right) showed less pronounced histologic changes compared to mice sensitized by both IP and EC route (left). (C) Mice sensitized by EC route alone (white) showed less robust elevations in tissue IL-4 mRNA and circulating OVA-specific IgE compared to mice sensitized by both IP and EC route (black).

Notes: Data are presented as mean ± SEM of five (days 1, 7) or nine (day 3) IP + EC sensitized mice and three (day 7) or four (day 1, 3) EC sensitized mice.

Abbreviations: DC, dendritic cell; EC, endothelial cell; OVA, ovalbumin; IFN, interferon; IL, interleukin; IP, intraperitoneal; mRNA, messenger ribonucleic acid; ND, no data; SEM, standard error of the mean.