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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Nat Methods. 2013 Oct 6;10(12):1213–1218. doi: 10.1038/nmeth.2688

Figure 1. ATAC-seq is a sensitive, accurate probe of open chromatin state.

Figure 1

(a) ATAC-seq reaction schematic. Transposase (green), loaded with sequencing adapters (red and blue), inserts only in regions of open chromatin (nucleosomes in grey) and generates sequencing library fragments that can be PCR amplified. (b) Approximate reported input material and sample preparation time requirements for genome-wide methods of open chromatin analysis. (c) A comparison of ATAC-seq to other open chromatin assays at a locus in GM12878 lymphoblastoid cells displaying high concordance. Lower ATAC-seq track was generated from 500 FACS-sorted cells.