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Effects of the z-position of the objective lens on a transmitted light image of a mitochondrion. A) Transmitted light images of a mitochondrion at different z-positions of the objective lens. We set z = 0 at an appropriate position. Bar, 1 μm. B) Dependence of Rplane on the z-position. Rplanes of 3 different mitochondria (a, b, c) in the same microscopic field are shown. Definition of Rplane is written in “Methods” section
