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Dependence of PΔ(0.02) upon addition of hypotonic solution on Tinit. All mitochondria images recognized as single mitochondria were analyzed. A) The distributions of Tinits. Mitochondria with ΔT > 0.02 and mitochondria with ΔT < 0.02 are indicated separately. B) P0.02(Tinit,th) and the percentage of mitochondria with Tinits < Tinit,th indicated in the horizontal axis. Definitions of ΔT, PΔ(ΔTth), Tinit, Tinit,th, and P0.02(Tinit,th) are described in the “Results” section.
