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. Author manuscript; available in PMC: 2015 Feb 6.
Published in final edited form as: Mol Cell. 2014 Feb 6;53(3):484–497. doi: 10.1016/j.molcel.2014.01.011

Figure 4. TDRD3-TOP3B complex regulates c-MYC gene expression.

Figure 4

(A) The mRNA levels of several TDRD3 target genes, in control and TDRD3 knockdown MCF7 cells, were detected by RT-qPCR. Error bars represent standard deviation calculated from triplicate qPCR reactions.

(B) Raw reads of the ChIP-seq tracings for three genes – c-MYC, MYADM, and EGR1 – demonstrating the enrichment of TDRD3 at c-MYC, but not negative controls. The regions that were subjected to qPCR in (C) and (D) are depicted.

(C) Chromatin immunoprecipitation experiment was performed in control and TDRD3 knockdown MCF7 cells using control IgG and αTDRD3 antibodies. The ChIP DNA was analyzed by qPCR with primers for the indicated region (B).

(D) Chromatin fractions from MCF7 cells were immunoprecipitated with αPRMT1, αCARM1, αPRMT6, H4R3me2a, H3R17me2a, H3R2me2a, αTDRD3, αTOP3B and control antibodies. The ChIP DNA was analyzed by qPCR with primers for the indicated region (B). * = p-value of 0.01 to 0.05, and ** = p-value of 0.001 to 0.01.

See also Figure S4.