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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cancer Res. 2013 Oct 29;73(24):7301–7312. doi: 10.1158/0008-5472.CAN-13-1897

Figure 4. YEATS4 knockdown impairs growth and induces senescence.

Figure 4

shRNA targeting YEATS4 significantly reduces (A) mRNA expression and (B) protein levels in all cell lines relative to controls (PLKO). GAPDH was used as a loading control. (C) Viability of cell lines with knockdown (shY4) relative to controls as measured by MTT. (D) Colony formation ability of shY4 cell lines relative to controls. (E) Quantification of cellular senescence based of β-Gal staining. Values reported as mean ± SEM of triplicate experiments.* p<0.05, ** p<0.01, *** p<0.001, Student’s t-test of shY4 cells relative to PLKO. (F,G) Effect of YEATS4 knockdown on tumor growth in mice injected with H1993 or H1355 PLKO and shY4 cells. Error bars indicate SEM of each group of 10 mice, * p<0.05