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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cancer Res. 2013 Oct 29;73(24):7301–7312. doi: 10.1158/0008-5472.CAN-13-1897

Figure 5. YEATS4 alters p21 and p53 protein levels.

Figure 5

(A) Knockdown of YEATS4 increases expression of p21 in cell lines with YEATS4 amplification and increases p53 in all lines that express p53. (B) Overexpression of YEATS4 reduces p14 and p21 levels in both HBEC lines, and p53 only in the HBEC KT line. Dose-response curves of HBEC KT (C) and H1993 (D) cells treated with 2-fold dilutions of cisplatin for 72 hours. Viability is shown as a proportion of treated cells against untreated controls (mean ± SEM of triplicate experiments). (E) Immunoblot of PLKO and shY4 cell lines treated with 40μm of Cisplatin for 0, 24 or 48 hours. Cisplatin treatment induces apoptosis as measured by the increase in cleaved PARP, p53 and phosphorylated p53 (Ser15). GAPDH was used as a loading control for all blots.