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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mol Microbiol. 2014 Jan 27;91(5):996–1008. doi: 10.1111/mmi.12510

Fig. 1.

Fig. 1

CcmFGH attaches heme to cytochrome c in the absence of CcmABCDE. (A) Heme staining of BPER cell extracts showing synthesis of 24-kDa holocytochrome c4:His6 as a function of expression of separate Ccm components, as indicated. (B) Heme staining and (C) anti-cytochrome c2 immunoblot of BPER cell extracts showing synthesis of 106-kDa holocytochrome c2:Pho by CcmFGH as a function of induction with IPTG and/or arabinose. One hundred μg total protein was loaded in each lane. MW, molecular weight standards (lanes 1).