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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mol Microbiol. 2014 Jan 27;91(5):996–1008. doi: 10.1111/mmi.12510

Fig. 5.

Fig. 5

Effect of substitutions at conserved His residues in CcmF on holocytochrome c formation in the absence of CcmABCDE. (A) Heme staining of purified holocytochrome c4 assembled by CcmFGH-only (“WT”) and site-directed variants of CcmF. (B) Quantification of the results of heme staining (24 kDa holocytochrome c4) from purified fractions from three independent experiments. (C) Heme staining of purified holocytochrome c4 assembled by CcmFGH-only (“WT”) and the indicated site-directed variants at CcmF P-His1 (upper panel) or P-His2 (lower panel). (D) Quantification of the results of heme staining from purified fractions from three independent experiments. For (B) and (D), synthesis of holocytochrome c4 is relative to CcmFGH-only (“WT”), which has been set at 100 %. Error bars denote standard deviation. ND, none detected.