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. 2014 Mar 19;9(3):e83895. doi: 10.1371/journal.pone.0083895

Figure 5. MKL1 regulates hypoxia-induced collagen transcription in smooth muscle cells.

Figure 5

(A) Col1a1 or col1a2 promoter luciferase construct was transfected into A10 cells with MKL1 expression construct followed by exposure to 1% O2 for 24 hours. Luciferase activities were expressed as relative luciferase unit (RLU). (B) Col1a1 or col1a2 promoter luciferase construct was transfected into A10 cells with shRNA plasmid targeting MKL1 or a control shRNA plasmid (nontarget) followed by exposure to 1% O2 for 24 hours. Luciferase activities were expressed as RLU. (C, D) A10 (C) or primary human pulmonary arterial smooth muscle (D) cells were transfected with MKL1 specific or control siRNA (SCR) followed by exposure to 1% O2 for 24 hours. Expression of collagen type I genes was measured by qPCR.