Figure 7. Effect of mMP on endothelial cell monolayer.

Co-incubation of mMP with resting endothelial cells induced an increase in monolayer impedance (A). Similarly, co-culturing mMP onto pre-stimulated endothelial cells induced raised impedance of the endothelial monolayer whereas stimulation with TNF alone (or with SN) decreased TEER (B). The SN did not have any effect. After overnight co-culture, mMP did not alter the passage of FITC-dextran through either resting (C) or TNF-primed endothelial monolayers (D) over 4 hours. Data shown are representative of three independent experiments. FITC-dextran permeability assays were performed in triplicates and expressed as means ± SD.