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. 2014 Mar 19;9(3):e90807. doi: 10.1371/journal.pone.0090807

Figure 4. CHYS blocks activation of the TGF-β/Smad signaling pathway in the diabetic rats.

Figure 4

Western blot (A) and quantitative analysis of renal TGF-β (C), TβR II (D), p-TβR I and TβR I (E) expression, respectively. Real-time PCR for renal TGF-β1 mRNA expression (B). Western blot (F) and quantitative analysis of phospho-Smad3, Smad3 (H) and Smad7 (I) expression, respectively. Real-time PCR for renal Smad7 mRNA expression (G). Data represent mean ± SE for groups of 14 rats. *P<0.05, **P<0.01, ***P<0.001 vs. sham group; # P<0.05, ## P<0.01, ### P<0.001 vs. DN group.