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. 2014 Mar 19;9(3):e91849. doi: 10.1371/journal.pone.0091849

Figure 2. EGFL7 knockdown does not influence VEGFR2 phosphorylation or neuropilin 1 expression.

Figure 2

A, Mouse eye cups of each group were treated with EGFL7 or control siRNA after embedding them in Matrigel. Samples were cultured in VEGF (25 ng/ml) containing medium. At 3 days after knockdown of EGFL7, endothelial cells were collected using anti-mouse CD31 antibody-coated magnetic beads (see Figure S4). The amounts of p-VEGFR2, neuropilin 1 and EGFL7 were examined by Western blotting. B, Densitometry of p-VEGFR2 in panel A. C, Densitometry of neuropilin 1 in panel A. D, Densitometry of EGFL7 in panel A. ANOVA Statistical analysis performed. (n = 3) *, P<0.01. **, P<0.05. NS, not significant. *, P<0.01. NS, not significant.