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. 2014 Mar 19;9(3):e91849. doi: 10.1371/journal.pone.0091849

Figure 3. EGFL7 mediates VEGF-induced activation of Akt and ERK(1/2).

Figure 3

A, Mouse eye cups of each group were treated with EGFL7 or control siRNA after embedding them in Matrigel. Samples were cultured in VEGF (25 ng/ml) containing medium. At 3, 5 and 7 days after knockdown of EGFL7, endothelial cells were collected using anti-mouse CD31 antibody-coated magnetic beads. The amounts of (p-)Akt, p-ERK(1/2), EGFL7 and α-SMA in isolated cells were examined by Western blotting. B, Densitometry of p-Akt in panel A. C, Densitometry of p-ERK(1/2) in panel A. D, Densitometry of EGFL7 in panel A. ANOVA Statistical analysis performed. (n = 3) *, P<0.01. **, P<0.05. NS, not significant. *, P<0.01. **, P<0.05. NS, not significant.