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. 2014 Mar 19;9(3):e91712. doi: 10.1371/journal.pone.0091712

Figure 3. Construction and GOI expression of HR vectors.

Figure 3

(A) Schematic diagram of HR vectors. Three HR vectors were constructed from the TNFR-Fc expression vector. Fragments of the SEGFP expression cassette with different lengths were used for HR in the upstream and downstream sequences of the TNFR-Fc expression cassette. (B) HR vectors were transiently transfected into CHO cells using the TNFR-Fc expression vector as the control. The titers and Qp of TNFR-Fc were measured and compared among these vectors.