Figure 3. Determination of Th cell subset proportion by determining their specific cytokines IL17 (A), IFNG (B), IL4 (C), and IL10 (D) by flow cytometry in PBMCs.

The Th2/Th1 ratio (E) was further calculated. The samples were collected from AR patients (open circles) and the controls (open triangles). Connecting lines indicate data obtained in the same subject before and after SIT treatment. Medians are shown by horizontal dashed lines. Statistical analyses were performed by Wilcoxon matched pairs sign rank test for paired comparison (SIT-untreated vs. SIT-treated patients); while Mann-Whiney two-tailed test for unpaired comparison (SIT-untreated or SIT-treated vs. control subjects).