Figure 4. Quantitative determination of mRNA by real-time RT PCR for IL17 (A) and RORC (B) in PBMCs and evaluation of protein production by ELISA for IL17 (C) in supernatant after HDM challenge.

The samples were collected from SIT-untreated AR patients (open circles), SIT-treated AR patients (close circles) and the controls (open triangles). Connecting lines indicate data obtained in the same subject before and after HDM stimulation. Statistical analyses were performed by Wilcoxon matched pairs sign rank test. “NS” indicates not significantly different.