Figure 5. Determination of Th cell subset proportion by determining their specific cytokines IL17 (A), IFNG (B), IL4 (C), and IL10 (D) by flow cytometry in PBMCs after HDM challenge.

The samples were collected from SIT-untreated AR patients (open circles), SIT-treated AR patients (close circles) and the controls (open triangles). Connecting lines indicate data obtained in the same subject before and after HDM stimulation. Statistical analyses were performed by Wilcoxon matched pairs sign rank test. “NS” indicates not significantly different.