Figure 6. Identification of the cleavage products from plasminogen digested with Sericase.

A) The digest run on a Tris Glycine 4–20% gel. The white and gray arrows show bands excised for N-terminal sequencing. The N-terminal cleavage sites are indicated as amino acid positions of Plg. B) Schematic overview of Plg and the cleavage sites for Sericase; PAP is preactivation peptide. C) Calculated and observed (by LC-MS) masses of the plasminogen derivates containing the catalytic domain.