Figure 5. Regulation of p53 family signaling by propranolol.
A, p53 and pHDM2 western immunoblots of lysastes from SK-N-SH cells treated with propranolol (100 μM) for the indicated durations. B-E, TAp73β, NOXA and p57kip2, COX-2, western immunoblots of lysates from SK-N-AS cells treated with 150 μM of propranolol for the indicated durations. F, alamarBlue assays were performed on SK-N-AS cells treated with increasing doses of propranolol and Celecoxib (CXB) at a ratio of 2:1 (150μM propranolol: 75 μM CXB) for 48hr. Results are expressed as percentage compared to controls. The combination index (CI) was determined based on the Chou-Talalay method is shown on the top of the bar representing combination treatment * p<0.0001