Figure 4. Chloroquine enhances FTS-induced inhibition of anchorage-independent growth.

(A) Panc-1 cells (2,700 cells/ well) and (B) HCT-116 cells (5,000 cells/well) were grown in soft agar for 13 or 11 days, respectively, in the presence of FTS, with or without chloroquine (CQ), at the indicated concentrations. Colonies were then stained as described in Materials and Methods. Left panels, photomicrographs of typical wells. Right panels, number of colonies (>0.01 mm²) is presented for each treatment as mean ± SD (n=6; **, p < 0.01 compared to each treatment alone).