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Ring-stage parasites from HbAA and HbAS (or HbAC) children were cultured to trophozoites (a), and then purified by magnetic column and inoculated into wildtype donor RBCs (b). After invasion and maturation to trophozoites expressing PfEMP1 (c), parasites from HbAS or HbAC children were compared for binding to MVECs in parallel with those from HbAA children (d).
