Figure 3. Expression of K5 mRNA and protein.

(A) Time-dependent K5 protein (∼14 kDa) expression stimulated with 5 MBq/ml Na¹³¹I in Bac-hTERT-NIS-Egr1-K5-infected Hela cells was analyzed by Western blot. GAPDH was used as an internal control. Then Dose-dependent K5 protein expression stimulated with Na¹³¹I for 18 h in infected Hela cells was also extracted for Western blot analysis. (B) Total RNA samples from Hela cells were extracted after incubation with Bac-hTERT-NIS-Egr1-K5 and stimulation with or without 5 MBq/mL Na¹³¹I for 18 h. K5 mRNA expression normalized to the GAPDH endogenous reference was detected by qRT-PCR. Results are expressed as means ± SD of three independent experiments. *P<0.05. (C) Time-dependent K5 protein expression stimulated with 5 MBq/ml ¹³¹I in HUVECs infected with Bac-hTERT-NIS-Egr1-K5 was analyzed by Western blot. GAPDH was used as an internal control.