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. 2014 Mar 19;9(3):e92380. doi: 10.1371/journal.pone.0092380

Figure 4. DC transfer promotes enhanced CTL functions compared to peptide plus adjuvant vaccination.

Figure 4

Total splenocytes were isolated on day 6 following vaccination with unstimulated DC loaded with peptides gp33–41, gp276–286, and gp61–80 (unstim DC), CpG-matured, peptide-loaded DC (CpG DC), or peptides mixed with LPS and anti-CD40 (LPS+anti-CD40), as well as naive (unvaccinated) and LCMV infected controls. (A) IFN-γ and TNF-α expression in CD8+ T-cells were measured following peptide restimulation. (B) Splenocytes were stained with anti-CD8 antibody and gp33–41 or gp-34–41 tetramers. (C) gp33–41 and control AV peptide pulsed and CFSE labeled target cells were transfer into vaccinated mice on day 6 post immunization. 4 hrs later, splenocytes were harvested and examined by flow cytometry for specific lysis of targets. Results show means+/−S.D. from 3 mice per group and are representative of 3–5 independent experiments. **P<0.01; *P<0.05.