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. 2014 Mar 19;9(3):e92466. doi: 10.1371/journal.pone.0092466

Figure 5. S1P-induced IL-8 protein secretion is repressed by inhibitors of the p38 MAPK- and ERK-mediated pathways.

Figure 5

Growth-arrested ASM cells were pretreated for 30 min with vehicle, 1 μM SB203580, or 10 μM PD98059 to inhibit p38 MAPK and ERK, respectively. Cells were then stimulated with 1 μM S1P for 24 h. IL-8 protein was measured by ELISA and results expressed as % S1P-induced IL-8 secretion at 24 h. Statistical analysis was performed using the Student's unpaired t test, where * denotes significant inhibition (P<0.05). Data are mean+SEM values from n = 10 primary ASM cell lines.