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. 2014 Mar 19;9(3):e92447. doi: 10.1371/journal.pone.0092447

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© 2014 Lee et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Time course study of mRNA expression of MYL2, MYH3 and CCNA2 during MSCs differentiation. Cells cultured in differentiation media showed gradual increase in MYL2 and MYH3 expression until Day 18, but transient increase of CCNA2 at day 12 decreased at later stage of myogenesis. B) Evaluation of MYL2, MYH3, and CCNA2 gene expression by real time RT-PCR in MyoG_kd cells. Decreased MYL2 and MYH3 gene expression and increased CCNA2 expression was observed in MyoG_kd cells relative to MyoG_kd cells. Day 10 and MyoG_wd represents control, respectively (mean ±S.D., n = 3). p-value indicates the statistical significance of the data and different letters (a, b and c) in graph show significant differences among groups.