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. 2014 Mar 19;9(3):e92627. doi: 10.1371/journal.pone.0092627

Figure 3. Phosphorylation of Akt (Ser473) and Akt (Thr308) in B16F10 cells after melatonin with or without thapsigargin or tunicamycin treatment.

Figure 3

B16F10 cells were cultured with or without melatonin (0.1, 0.5, 1 mM) and/or thapsigargin (1 μM) for 6 hr or tunicamycin (5 μg/mL) for 16 hr. p-Akt (Ser473) and p-Akt (Thr308) were analyzed by Western blot (A). Relative amounts of p-Akt (Ser473) (B) and p-Akt (Thr308) (C) quantified as described in Materials and methods. Data are mean ± SD of three experiments. ***P<0.001 vs. untreated control; ### P<0.001, melatonin vs. melatonin with thapsigargin or tunicamycin.