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. 2014 Mar 19;9(3):e92580. doi: 10.1371/journal.pone.0092580

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The defined mixtures of wt A/Victoria/361/2011(H3N2) and MDV A/Leningrad/134/17/57(H2N2). RNAs were analyzed by pyrosequencing of NS gene. The signature nucleotides of wt are shown in red, MDV – in green. The analysis was carried out with (A) 100% wt, mixtures of wt:MDV - (B) 95∶5, (C) 90∶10, (D) 75∶25, (E) 50∶50, (F) 10∶90, (G) 5∶95) and (H) 100% of MDV RNA.