Table 1. Oligonucleotide primers used for RT-PCR amplification of MDV and H3N2 viruses.

Oligo ID	Gene	Nucleotide region	Direction	Sequence 5′ ->3′
NA-1246F	NA	1246–1268	Forward	AGCTGCATCAATMGGTGCTTTTA
NA-1334R	NA	1334–1353	Reverse	Biotin-ACCTGARGTGCCACAAAACA
NS-354F	NS	354–372	Forward	AATGGACCAGGCAATCATG
NS-458R	NS	436–458	Reverse	Biotin-TCTTCGGTGAAAGCCCTTAGTAA
M-787F	M	787–805	Forward	TGGGATCTTGCACTTGATA
M-873R	M	854–873	Reverse	Biotin-TCTTTTMAGRCCGTGTTT
PB1-38F	PB1	38–56	Forward	Biotin-CAGCGCAAAATGCCATAAG
PB1-219R	PB1	196–219	Reverse	TAGTGGTCCATCAATTGGGTTRAG
PB2-120F	PB2	120–143	Forward	AAAGAACCCGTCACTTAGGATGAA
PB2-308R	PB2	285–308	Reverse	Biotin-CCATTYCTATTCCACCATGTYACA
PA-66F	PA	66–88	Forward	AGAGTATGGRGAGGATCYGAAAA
PA-328R	PA	308–328	Reverse	Biotin-ACAAATCYGGYAGAAACTTCG
NP-36F	NP	36–56	Forward	Biotin-GATGGAAACTGATGGGGAWCG
NP-140R	NP	118–140	Reverse	AGTTCRGTGCACATTTGGATGTA