Table 2. Oligonucleotide primers used for RT-PCR amplification of MDV and H7N9 viruses.
| Oligo ID | Gene | Nucleotide region | Direction | Sequence 5′ ->3′ |
| NA-Len-UTR-F | NA | 1–19 (5′ UTR) | Forward | AGCAAAAGCAGGAGTGAAA |
| NA-N9-UTR-F | NA | 1–18 (5′ UTR) | Forward | AGCAAAAGCAGGGTCAAG |
| NA-H7N9-374R | NA | 353–374 | Reverse | Biotin-TCGCATGAMACATAAGRTTCTC |
| M-H7N9-787F | M | 787–805 | Forward | TGGGATMTTGCACTTGATA |
| M-H7N9-873R | M | 854–873 | Reverse | Biotin- CCTCTTTTCARACCGTRTTT |
| NS-H7N9-43F | NS | 43–61 | Forward | Biotin-CTTTGGCATGTCCGCAAAC |
| NS-H7N9-124R | NS | 105–124 | Reverse | ACTTCTGATCTCGGCGAAGC |
| NP-H7N9-1269F | NP | 1269–1286 | Forward | AACCATYATGGCAGCATT |
| NP-H7N9-1352R | NP | 1333–1352 | Reverse | Biotin-GCACYTTCCATCATYCTTAT |
| PA-H7N9-526F | PA | 526–548 | Forward | Biotin-TTCACCATAAGRCARGAAATGGC |
| PA-H7N9-600R | PA | 581–600 | Reverse | TGTTTCTTCGCCTCTTTCRG |
| PB1-H7N9-211F | PB1 | 211–233 | Forward | Biotin-GGACCAYTACCTGAGGACAAYGA |
| PB1-H7N9-293R | PB1 | 271–293 | Reverse | GATTCTTCAAGGAAAGCCATTGC |
| PB2-H7N9-27F | PB2 | 27–49 | Forward | Biotin-TYTGATGTCRCAGTCTCGCACTC |
| PB2-H7N9-160R | PB2 | 140–160 | Reverse | TCATTGCCATCATCCAYTTCA |