Table 6. Target sequences for genotyping by pyrosequencing of MDV and H7N9 viruses.

Virus	Gene	Nucleotides	Sequence 5′ ->3′
MDV	NA	21–35	AATAACAATTGGCTC*
A/Anhui/1/2013	NA	21–35	TC TATGCACTTCAGC
MDV	M	840–855	A TTATCGCTTCTTTAA
A/Anhui/1/2013	M	840–855	TTT ATCGTCGTTTTAA
MDV	NS	92–104	CGATCAAGGAATG**
A/Anhui/1/2013	NS	92–104	CGGTCTAGAAATG**
MDV	NP	1287–1301	C ACTGGGAATGCAGA
A/Anhui/1/2013	NP	1287–1301	T ACAGGAAATACTGA
MDV	PA	546–561	GAGGCCTCTGCTAGCC**
A/Anhui/1/2013	PA	546–561	TAGACCCCTGCTGGCC**
MDV	PB1	250–266	AGGACGCAGTCTGTTTG**
A/Anhui/1/2013	PB1	250–266	AATACACAATCCGTTTG**
MDV	PB2	117–133	GTGACGGGTTCTTTTCC**
A/Anhui/1/2013	PB2	117–133	GGGCAGGATTCTTCTCC**

*Signature nucleotides are shown in bold.

**The sequence is of the complementary strand.