Table 7. Genome composition of reassortants between MDV and wtl viruses identified by pyrosequencing.
Virus used for reassortment | Clone number | Reassortment step | Gene | origin | |||||
HA | NA | NS | M | NP | PA | PB1 | |||
A/Victoria/361/2011 (H3N2) | 6.4 | 1st cloning | wt | wt | MDV | MDV | MDV | MDV | MDV |
A/Ohio/02/2012 (H3N2) | 4.1 | 1st cloning | wt | wt | mix | mix | MDV | MDV | MDV |
48.5 | 2nd cloning | wt | wt | MDV | MDV | MDV | MDV | MDV | |
A/Texas/50/2012 (H3N2) | 4.1 | 1st cloning | wt | wt | mix | mix | mix | mix | mix |
46.2 | 2nd cloning | wt | wt | mix | MDV | MDV | MDV | MDV | |
467.6 | 3d cloning | wt | wt | mix | MDV | MDV | MDV | MDV | |
4678.2 | 4th cloning | wt | wt | MDV | MDV | MDV | MDV | MDV | |
A/Anhui/1/2013 (H7N9) | 2.3 | selective | wt | mix | mix | mix | mix | MDV | MDV |
28.3 | 1st cloning | wt | wt | MDV | MDV | MDV | MDV | MDV |